Genetic Transformation Of Genetic Dna - 877 Words

Genetic transformation occurs when an organism’s genetic makeup is altered due to the introduction of new genetic information which is then incorporated into the organism’s genome. In this lab the pGLO plasmid is introduced into E. Coli bacteria, and incorporates the genes which code for the GFP and beta lactamase to the bacteria’s genome which as a result will be modified. To test the effects of the plasmid, bacteria treated with the plasmid were grown on separate plates, the first containing LB nutrient broth and ampicillin, another containing LB nutrient broth and arabinose and another containing LB nutrient broth, ampicillin and arabinose. Two more plates were grown, one with LB nutrient broth and ampicillin and the other with only the LB broth, using cells that did not contain the plasmid. Since the lab was about genetic transformation, the goal was to find which plate would glow. It was found that the plates that were not exposed to the plasmid did not glow , and the plates containing LB and arabinose and LB, ampicillin and arabinose did glow. The plates containing ampicillin, the antibiotic that kills E. coli did not grow whereas the remaining plates at least had some growth. Introduction Transformation is the directed modification of a genome by the external application of DNA from a cell of different genotype (Griffiths and Miller).Bacterial transformation is the easiest type of transformation to create and study due to the single cellularity of bacteria and itsShow MoreRelatedHeritable Genetic Information In Escherichia Coli In Transformation1664 Words   |  7 PagesHeritable Genetic Information in Escherichia Coli in Transformation and Deoxyribonucleic Acid A report for Biology 2000 written by Alana Pay : 001196733 Introduction Griffin was a scientist that showed that there was a non-living component to heritability between organisms, although he had not yet discovered what exactly was the heritable component. However, during this time period, it was a common belief that the heritable material was contained within the protein of the organism, not the DNA thatRead MoreEssay on The Discovery Of The Structure Of DNA1526 Words   |  7 PagesJames Watson and Francis Crick discovered the structure of DNA, but only by drawing on the work of many scientists who came before them. (Maddox, 2003) In 1944, Oswald T. Avery, Colin M. MacLeod, and Maclyn McCarty published â€Å"Studies on the Chemical Nature of the Substance Inducing Transformation of Pneumococcal Types†, which was the first scientific work to identify DNA as the molecule that carried genetic information, and became a breakthrough at that time. (Avery, Macleod, McCarty, 1944) BeforeRead MoreLara Guvelioglu. Bi 108 E2. Nahomie Rodriguez-Sastre. 04/13/17.1632 Words   |  7 PagesBacteria Escherichia coli’s Genetic Transformation Using Vector Plasmid DNA pGLO Abstract The field of biotechnology involves the concept of genetic engineering, altering the DNA/genetic material of an organism using information from a different one. The process in which bacteria can obtain this manipulated genetic information from another source is called genetic transformation. The goal of this experiment was to genetically transform Escherichia coli bacteria’s DNA by inserting the vector pGLORead MoreThe Domestication of Animals Was the Percursor to Genetic Engineering706 Words   |  3 Pages The domestication of animals 30,000 years ago was the precursor to Genetic Engineering. Starting with the dog we have since been able to introduce desirable traits in all organisms. With the discovery of plasmids in the late 60’s we have been able to take genetic engineering even further. 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Gene cloning has four basic processes, namely cutting : the process of isolating the required starnd of DNA, modification : the process of altering the ends of the strand so that theyRead MoreGenetically Modified Organisms Are Safe And Should Be Used1226 Words   |  5 Pagesgenetically modified organisms. A genetically modified organism is living organisms whose genetic material has been artificially manipulated in a laboratory through genetic engineering. Although some see this as a bad thing, I personally feel that GMOs are safe and should be used routinely. Corn for example, is something that is used daily in the study of genetic modifications. One large example of the genetic modification is Bt corn. Corn has been around for many years and has served many useful purposesRead MoreGenetic Expression In Gene Expression801 Words   |  4 PagesIn gene expression DNA, the blueprint of life is transcribed into RNA, a nucleic acid that acts as a messenger carrying instructions that control the synthesis of proteins. Gene expression is vital to determining cell functions and exactly how much protein should be produced by the cell. The process in which the end proteins are modified can also be manipulated by researchers. The types of cells which are typically handled in laboratories include prokaryotes. Escherichia coli is a bacteria foundRead MoreEssay On Mutation1657 Words   |  7 PagesSpontaneous mutations occur during a normal cell process creating genetic changes (text p.206). There are varying types of spontaneous mutations including, silent, missense, nonsense, and frameshift mutations. How are these mutations the same and how are they different? Silent, missense, and nonsense mutations all occur when base substitutions happen (text p.206). A base substitution is when the wrong nucleotide is incorporated during DNA synthesis, this is the most common mutation (lecture). In a silent